Part:BBa_K4201009:Design
Arabidopsis thaliana Heat Shock Protein (AtHSP)
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 201
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 201
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 135
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 201
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 201
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 267
Design Notes
Codon optimization for Glycine max (soybean) is a unique design consideration for all of CU Boulder’s coding sequences including our AtHSP terminator. Codon optimization is the intentional use of specific codons for specific amino acids, dependent on what tRNAs are most abundant in the organism. While codon optimization is a common consideration for synthetic biologists, our sequences are unique for iGEM because they are intended for expression in soybeans.
Source
This Arabidopsis thaliana Heat Shock Protein terminator sequence originates from Arabidopsis thaliana. All of our DNA fragments are obtained via de novo synthesis by iGEM sponsors Twist Bioscience and Integrated DNA technologies.
References
1. Nagaya, S., Kawamura, K., Shinmyo, A. & Kato, K. The HSP Terminator of Arabidopsis thaliana Increases Gene Expression in Plant Cells. Plant Cell Physiol. 51, 328–332 (2010).
2. Hirai, T. et al. The HSP Terminator of Arabidopsis thaliana Induces a High Level of Miraculin Accumulation in Transgenic Tomatoes. J. Agric. Food Chem. 59, 9942–9949 (2011).
3. Rajan, V. B. V. & D’Silva, P. Arabidopsis thaliana J-class heat shock proteins: cellular stress sensors. Funct. Integr. Genomics 9, 433–446 (2009).