Terminator
AtHSP

Part:BBa_K4201009:Design

Designed by: Maya Li Nelson   Group: iGEM22_CU-Boulder   (2022-10-01)


Arabidopsis thaliana Heat Shock Protein (AtHSP)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 201
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 201
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 135
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 201
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 201
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 267


Design Notes

Codon optimization for Glycine max (soybean) is a unique design consideration for all of CU Boulder’s coding sequences including our AtHSP terminator. Codon optimization is the intentional use of specific codons for specific amino acids, dependent on what tRNAs are most abundant in the organism. While codon optimization is a common consideration for synthetic biologists, our sequences are unique for iGEM because they are intended for expression in soybeans.


Source

This Arabidopsis thaliana Heat Shock Protein terminator sequence originates from Arabidopsis thaliana. All of our DNA fragments are obtained via de novo synthesis by iGEM sponsors Twist Bioscience and Integrated DNA technologies.

References

1. Nagaya, S., Kawamura, K., Shinmyo, A. & Kato, K. The HSP Terminator of Arabidopsis thaliana Increases Gene Expression in Plant Cells. Plant Cell Physiol. 51, 328–332 (2010).
2. Hirai, T. et al. The HSP Terminator of Arabidopsis thaliana Induces a High Level of Miraculin Accumulation in Transgenic Tomatoes. J. Agric. Food Chem. 59, 9942–9949 (2011).
3. Rajan, V. B. V. & D’Silva, P. Arabidopsis thaliana J-class heat shock proteins: cellular stress sensors. Funct. Integr. Genomics 9, 433–446 (2009).